α-casein

Important role in determination of the surface properties of the casein micelles.
Mw: α-casein=24,500,

β-casein

Important role in determination of the surface properties of the casein micelles.
Mw: β-casein =25,000

ovalbumin

Storage protein of egg white
Mw: 45,000

pepsin

An enzyme that breaks down proteins in foods in stomach
Mw: 41,000

Image

1 α-casein
2 α-casein partially dephosphorylated
3 α-casein dephosphorylated

4 β-casein
5 β-casein partially dephosphorylated
6 β-casein dephosphorylated

7 molecular weight markedr

8 ovalbumin
9 ovalbumin dephosphorylated

10 pepsin
11 pepsin dephosphorylated

Mn²⁺– or Zn²⁺–Phos-tag SDS-PAGE of typical phosphorylated proteins, which are available commercially.

These proteins are useful for checking Phos-tag gel system in your lab.

Related data

α-casein，α-casein，ovalbumin

ovalbumin

pepsin

 

Reference	Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Phos-tag SDS-PAGE systems for phosphorylation　profiling of proteins with a wide range of molecular　masses under neutral pH conditions Proteomics, 12, 192-202 (2012)  10.1002/pmic.201100524
Composition of gel	1. Mn2+–Phos-tag:　100µM   Polyacrylamide: 10%(w/v) 2. Zn2+–Phos-tag:　100µM   Polyacrylamide: 8%(w/v) 3. Zn2+–Phos-tag:　100µM   Polyacrylamide: 12%(w/v)
Detection	CBB stain

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